The Australian Signals Directorate’s Australian Cyber Security Centre (ASD’s ACSC) has developed this guidance to help government, critical infrastructure providers and large enterprises defend against cyber attacks that involve the use of artificial intelligence (AI). Malicious actors may use AI to discover vulnerabilities, develop exploits within hours, target many victims at once - especially for internet-facing services such as websites, exploit a chain of lower severity vulnerabilities to cause high severity impact, and rapidly progress an attack from initial compromise to data theft, extortion or disruption.